This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
Immunogen
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
TUBB3
Reaktivität: Human
WB, ELISA, IHC
Wirt: Maus
Monoclonal
unconjugated
Applikationshinweise
Immunohistochemistry on Frozen and Paraffin Sections. Immunocytotochemistry on Fixed and Permeabilized cells. Suggested working dilution is 1/40. The conjugate was also successfully used on FFPE Sections using Confocal Microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).
Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).
Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).
CDCBM antikoerper, CFEOM3A antikoerper, TUBB4 antikoerper, beta-4 antikoerper, TUBB3 antikoerper, 3200002H15Rik antikoerper, M(beta)3 antikoerper, M(beta)6 antikoerper, tubb3 antikoerper, tubulin beta 3 class III antikoerper, tubulin, beta 3 class III antikoerper, tubulin beta 3 class III L homeolog antikoerper, beta-tubulin 3 antikoerper, tubulin beta-3 chain antikoerper, beta tubulin 3 antikoerper, TUBB3 antikoerper, Tubb3 antikoerper, tubb3 antikoerper, tubb3.L antikoerper, LOC100581245 antikoerper, tub3 antikoerper
Hintergrund
The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III