C3 Antikörper

Produktnummer ABIN111065

Produktdetails anti-C3 Antikörper

Target: C3 (Complement Component 3 (C3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Detects C3b deposits in tissue, on cells, on microorganisms and in immune complexes. Does not react with C3a or C3d. Inhibits hemolytic function of C3. It should be used with positive control anti C3b-α (clone H206, cat.no. BM5029), and negative control anti C3a (clone H13, cat.no. BM2131). Activated, deposited C3b bears 3b-α and -ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with clone H13.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Protein A affinity chromatography

Immunogen: Human complement component C3

Klon: H11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot. Immunohistochemistry on frozen tissue (Dilution 1: 10, Incubation time: 1h at RT). Successful use on paraffin embedded tissue was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL dist. water.

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu C3

Target: C3 (Complement Component 3 (C3))

Andere Bezeichnung: Complement C3 (C3 Produkte)

Hintergrund: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gen-ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling