VRADSERQNQ EYQRLMD Remarks: The epitope has been localized on amino acids 352-368 of the alpha-helical fragment.
Spezifität
This antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular Ca.! Recognizes a Mr 40,000 polypeptide (Cytokeratin 19) of Human glandular epithelia. Positive on the following cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Tumors Specifically Detected: All tested adenocarcinoma, cholangio ca. of liver, renal cell ca., transitional cell ca. of the bladder, ovary ca., squamous cell ca. of cervix, bronchus and lung (intermediate type), mesothelioma, carcinoid tumor of bronchus, breast ca., thymoma.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, Rat, Fish, Bovine.
Aufreinigung
Affinity Chromatography on Protein A .
Immunogen
Keratin K19 of Mr 40000 from cultured Human MCF-7 cells
ELISA. Western blot. Cytological Material. Immunohistochemistry on Frozen Sections: Dilution Buffer: Dilute 1/10 with PBS, pH 7.4, for use in Immunohistochemistry. Incubation Time: 1 hour at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL distilled water.
Buffer
PBS pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. This reagent is not sterile, but can be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.45µ Millipore filter (or equivalent).
Lagerung
-20 °C
Informationen zur Lagerung
Store at -20 °C or below before reconstitution. Reconstitute with 0.5 ml of cold distilled water. After reconstitution, aliquot and freeze unused portions in volumes appropriate for single usage.
Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19