CPA1 Antikörper (APC)

Produktnummer ABIN1112254

Produktdetails anti-CPA1 Antikörper (APC)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CPA1 Antikörper ist konjugiert mit APC

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Produktmerkmale: Monoclonal Mouse Anti-Human Carboxypeptidase A APC for identification of Carboxypeptidase A. CA2 is a mouse monoclonal antibody specific for mast cell carboxypeptidase.

Klon: CA2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Allophycocyanin (APC), Abs/Em: 651/662 nm.

Probennahme: 1. Pipette 50 µl of sample to be analysed (up to 10^6 cells) into each tube. 2. For each sample, add an appropriate volume of conjugated antibody (20 µl) directed to the cell surface antigen of interest and the appropriate isotype control. Incubate for 15 minutes in the dark at room temperature. (This step is only necessary is you want to perform a direct immunofluorescence staining for a cell surface antigen) 3. Add 100 µL of Intracell Reagent A, (Fixative), to each tube. Mix gently. 4. Incubate for 15 minutes at room temperature. 5. Wash once in 2 ml PBS Working Solution 1X. 6. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 7. Add 100 µL of Intracell Reagent B (Permeabilization), to each tube. 8. Add the appropriate volume of CPA APC for the intracellular antigen and the appropriate isotype control. 9. Incubate for 15 minutes in the dark at room temperature. 10. Wash once in 2 ml PBS Working Solution 1X. 11. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 12. Resuspend the cell pellet in 0,5 ml of 1% paraformaldehyde solution or an appropriate fluid for flow cytometric use, and store in the dark at 2-8 °C. Fixed cells should be analyzed within 24 hours.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing stabilizer solution, PBS 20 mM and 0,09% Sodium azide, pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Referenzen für anti-CPA1 Antikörper (APC) (ABIN1112254)

Austin, Trowsdale, Rudd, Bodmer, Feldmann, Lamb: "Functional expression of HLA-DP genes transfected into mouse fibroblasts." in: Nature, Vol. 313, Issue 5997, pp. 61-4, (1985) (PubMed).

Royston, Omary, Trowbridge: "Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia." in: Transplantation proceedings, Vol. 13, Issue 1 Pt 2, pp. 761-6, (1981) (PubMed).

Details zu CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Andere Bezeichnung: CPA (CPA1 Produkte)

Synonyme: cpa1 antikoerper, cbpa1 antikoerper, CPA antikoerper, CPA1 antikoerper, 0910001L12Rik antikoerper, Cpa antikoerper, carboxypeptidase A1 antikoerper, pancreatic carboxypeptidase A1 antikoerper, Carboxypeptidase A1 antikoerper, carboxypeptidase A1-like antikoerper, carboxypeptidase a1 antikoerper, carboxypeptidase A1, pancreatic antikoerper, CpipJ_CPIJ007169 antikoerper, CpipJ_CPIJ010802 antikoerper, CpipJ_CPIJ010805 antikoerper, MCYG_06789 antikoerper, MGYG_03840 antikoerper, cpa1 antikoerper, cbpa1 antikoerper, LOC100224866 antikoerper, CPA1 antikoerper, Cpa1 antikoerper