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MBP Tag Antikörper

WB Wirt: Maus unconjugated
Produktnummer ABIN1112992
  • Target
    MBP Tag
    Wirt
    • 12
    • 5
    Maus
    Konjugat
    • 15
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 15
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB)
    Immunogen
    Recombinant MBP protein with full length.
    Isotyp
    IgG1
  • Applikationshinweise
    WB (1:1000-10,000). Other applications have not been tested. The optimal dilutions should be determined by end user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
    Konservierungsmittel
    Sodium azide, Thimerosal (Merthiolate)
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
  • Target
    MBP Tag
    Substanzklasse
    Tag
    Hintergrund
    Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
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