MBP Tag Antikörper
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- Target
- MBP Tag
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Wirt
- Maus
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Konjugat
- Unkonjugiert
-
Applikation
- Western Blotting (WB)
- Immunogen
- Recombinant MBP protein with full length.
- Isotyp
- IgG1
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anti-MBP Tag antibody
WB, ELISA, IP Wirt: Kaninchen Polyclonal unconjugated
anti-MBP Tag antibodyWB Wirt: Kaninchen Polyclonal RB55937 unconjugated
anti-MBP Tag antibodyWB, ELISA Wirt: Maus Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB Wirt: Maus Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA Wirt: Maus Monoclonal 5B10B11 unconjugated
anti-MBP Tag antibodyWB, EIA Wirt: Maus Monoclonal 6-00E-004 unconjugated
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- Applikationshinweise
- WB (1:1000-10,000). Other applications have not been tested. The optimal dilutions should be determined by end user.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Buffer
- PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
- Konservierungsmittel
- Sodium azide, Thimerosal (Merthiolate)
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
-
- Target
- MBP Tag
- Substanzklasse
- Tag
- Hintergrund
- Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
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