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Clathrin Heavy Chain (CLTC) (Heavy Chain) Antikörper

CLTC Reaktivität: Human, Ratte, Rind (Kuh), Schwein, Amphibien WB, IHC (fro) Wirt: Maus Monoclonal CHC5-9 unconjugated

Produktnummer ABIN111496

Datenblatt (PDF)

Target Alle Clathrin Heavy Chain (CLTC) Antikörper anzeigen

Clathrin Heavy Chain (CLTC)
Bindungsspezifität
14

12

7

4

4

4

3

2

2

2

2

1

1

1

1

1
Heavy Chain
Reaktivität
88

55

38

7

6

5

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Human, Ratte, Rind (Kuh), Schwein, Amphibien
Wirt
65

18

5

1
Maus
Passende Sekundärantikörper
Klonalität
63

26
Monoklonal
Konjugat
33

6

4

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Unkonjugiert
Applikation
64

22

20

19

15

13

13

8

5

4

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität

Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Kreuzreaktivität (Details)

Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.

Aufreinigung

Gel filtration

Immunogen

Coated vesicles (Clathrin) of Bovine brain.

Klon

CHC5-9

Isotyp

IgM

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anti-Clathrin Heavy Chain (CLTC) antibody
CLTC Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein WB, ELISA, FACS, IP, ICC Wirt: Maus Monoclonal BF-06 unconjugated

anti-Clathrin Heavy Chain (CLTC) antibody
CLTC Reaktivität: Human, Maus, Ratte WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Clathrin Heavy Chain (CLTC) (N-Term) antibody
CLTC Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Affe, Avian, Huhn, Pferd, Kaninchen, Cat, Esel, Ziege, Meerschweinchen, Hamster, Schaf WB, IF Wirt: Ziege Polyclonal unconjugated

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CLTC Reaktivität: Human WB, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB24434 unconjugated

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CLTC Reaktivität: Human WB, ELISA, PLA Wirt: Maus Monoclonal 2E5 unconjugated

anti-Clathrin Heavy Chain (CLTC) (AA 1617-1630) antibody
CLTC Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Affe, Xenopus laevis, Huhn, Pferd, Kaninchen, Fledermaus, Zebrafisch (Danio rerio) IHC, IHC (p) Wirt: Ziege Polyclonal unconjugated

anti-Clathrin Heavy Chain (CLTC) (AA 851-990) antibody
CLTC Reaktivität: Human ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Clathrin Heavy Chain (CLTC) (AA 401-500) antibody
CLTC Reaktivität: Human, Maus WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Clathrin Heavy Chain (CLTC) (AA 232-340) antibody
CLTC Reaktivität: Human WB, ELISA Wirt: Maus Polyclonal unconjugated

Applikationshinweise

Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Rekonstitution

Restore with 1 mL distilled water.

Lagerung

4 °C

Informationen zur Lagerung

Store the antibody undiluted at 2-8 °C.
Target

Clathrin Heavy Chain (CLTC)

Andere Bezeichnung

Clathrin Heavy Chain (CLTC Produkte)

Hintergrund

Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

Gen-ID

281080

NCBI Accession

NP_776448

UniProt

P49951