Clathrin Heavy Chain (CLTC) (Heavy Chain) Antikörper

Produktnummer ABIN111496

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in WB und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Rind (Kuh), Schwein und Amphibien.

Kurzübersicht für Clathrin Heavy Chain (CLTC) (Heavy Chain) Antikörper (ABIN111496)

Target: Clathrin Heavy Chain (CLTC)

Reaktivität: Human, Ratte, Rind (Kuh), Schwein, Amphibien

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: CHC5-9

Produktdetails

Bindungsspezifität: Heavy Chain

Spezifität: Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.

Aufreinigung: Gel filtration

Immunogen: Coated vesicles (Clathrin) of Bovine brain.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL distilled water.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Antigendetails

Target: Clathrin Heavy Chain (CLTC)

Andere Bezeichnung: Clathrin Heavy Chain

Hintergrund: Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

Gen-ID: 281080

NCBI Accession: NP_776448

UniProt: P49951