Macrophages Antikörper

Produktnummer ABIN111839

Produktdetails

Target: Macrophages

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Ki-M2R is a reliable tool for indicating macrophage differentiation in vivo and in vitro. This antibody recognizes typical tissue macrophages and specifically discriminates between monocytes (Ki-M2R-) and macrophages (Ki-M2R+). It stains all resident macrophages including those of bone marrow, lymphatic sinuses, lymphoid follicles, splenic red pulp, and Kupffer cells in the liver. Langerhans cells, interdigitating reticulum cells, and dendritic reticulum cells of lymphoid follicles are not stained. Ki-M2R recognises a surface antigen of 45 kDa molecular weight as a single band under reducing conditions. The epitope has not been further characterized. Antigen Distribution: Isolated Cells: In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoetic cells and megacaryocytes are negative. Immature accessory cells such as dendritic or interdigitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material. Tissue Sections: Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kupffer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50 % of alveolar macrophages) and connective tissue (histiocytes) are positively stained. Plasma cells (excluding stimulated monocytes) as well as sinus lining cells are negative.

Kreuzreaktivität (Details): Species reactivity (tested):Rat (Mature Macrophages).

Aufreinigung: Affinity Chromatography.

Immunogen: Rat peritoneal Macrophages

Klon: Ki-M2R

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry: Frozen Sections: 0.5 μg/mL (1/400)Paraffin Sections: 5 μg/mL (1/40)(Proteinase K pretreatment for antigen retrieval isrecommended). Suggested Positive Control: Rat Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1 h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 0.5 mL distilled water to a concentration of 0.2 mg/mL.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen

Ramaglia, King, Nourallah, Wolterman, de Jonge, Ramkema, Vigar, van der Wetering, Morgan, Troost, Baas: "The membrane attack complex of the complement system is essential for rapid Wallerian degeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 29, pp. 7663-72, (2007) (PubMed).

Antigendetails

Target: Macrophages

Hintergrund: Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).Synonyms: Macrophage marker