Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.
Aufreinigung
Affinity Chromatography
Immunogen
Purified rat surfactant protein D from bronchoalveolar lavage. Remarks: The antigen epitope is localized in the Lectin domain of SP-D.
SFTPD
Reaktivität: Human
WB, ELISA
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
Immunohistochemistry (IHC) on Frozen sections (2 μg/mL (1/200) for rat cryosections) andParaffin sections. ELISA. Western Blots. Suggested positive control: Rat lung. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 0.5 mL distilled water.
Konzentration
0.4 mg/mL
Buffer
PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
COLEC7 antikoerper, PSP-D antikoerper, SFTP4 antikoerper, SP-D antikoerper, BDE antikoerper, BDSD antikoerper, HOX4I antikoerper, SPD antikoerper, AI573415 antikoerper, Sftp4 antikoerper, surfactant protein D antikoerper, homeobox D13 antikoerper, surfactant associated protein D antikoerper, SFTPD antikoerper, HOXD13 antikoerper, Sftpd antikoerper, SP-D antikoerper
Hintergrund
Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D