B-Cells Antikörper
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- Target
- B-Cells
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Reaktivität
- Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Affinity Chromatography
- Immunogen
- Rat Lymphocytes Remarks: The epitope has not been further characterized.
- Klon
- Ki-B1R
- Isotyp
- IgG1
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- Applikationshinweise
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Immunohistochemistry on Frozen Sections: 1 μg/mL (1/100). Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/10). No antigen retrievalrequired, microwave treatment may enhance the reactivity. Suggested Positive Control: Rat spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore with 0.5 mL distilled water.
- Konzentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium azide as a preservative
- Konservierungsmittel
- Thimerosal (Merthiolate)
- Vorsichtsmaßnahmen
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- B-Cells
- Hintergrund
- B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.Synonyms: B cells, B-cell marker
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