Macrophages Antikörper

Produktnummer ABIN111867

Produktdetails

Target: Macrophages

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Species reactivity (tested):Mouse: Subpopulation of mature Macrophages.

Aufreinigung: Affinity Chromatography

Immunogen: Adherent bone marrow cells Remarks: The antigen is found in Mouse Monocytes and a subset of Macrophages.

Klon: ER-HR3

Isotyp: IgG2c

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 10 μg/mL (1/100)Paraffin sections: 50 μg/mL (1/20). Proteinase K pretreatment for antigen retrieval isrecommended. Has been described to work in FACS. Positive control: Mouse spleen. Antigen distribution on Isolated cells and Tissue sections: The antigen is found on up to70 % of circulating monocytes, all other leukocytes are ER-HR 3 negative. It is also found ona subpopulation (about 30 %) of bone marrow cells, mainly consisting of myeloid cells. In the adult mouse, the antigen is found on distinct subpopulations of resident tissuemacrophages in various organs. It is found on a subpopulation of the splenic red pulpmacrophages, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer'spatches and bone marrow. Epidermal Langerhans cells also express the antigen, whereasmacrophages in the connective tissue of the dermis and the gastrointestinal tract onlyscarcely express the ER-HR 3 related antigen. In the kidney, ER-HR 3 positive macrophagesbelong to the type 2 interstitial cells in the outer medulla which are negative with BM 8. Distinct ER-HR 3 positive macrophage subpopulations are found in various embryologicalorgans where hematopoietic islands occur, and where they are closely associated witherythrocyte precursor cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % Kathon, 5 mg/mL BSA

Konservierungsmittel: Kathon CG

Vorsichtsmaßnahmen: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Referenzen

Sartorius, DApice, Trovato, Cuccaro, Costa, De Leo, Marzullo, Biondo, DAuria, De Matteis, Ciccodicola, De Berardinis: "Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response." in: EMBO molecular medicine, Vol. 7, Issue 7, pp. 973-88, (2015) (PubMed).

Popovic, Wang, Papatriantafyllou, Kaya, Porubsky, Meisner, Bonrouhi, Burgdorf, Young, Schaefer, Gröne: "The proteoglycan biglycan enhances antigen-specific T cell activation potentially via MyD88 and TRIF pathways and triggers autoimmune perimyocarditis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 12, pp. 6217-26, (2011) (PubMed).

Hakroush, Moeller, Theilig, Kaissling, Sijmonsma, Jugold, Akeson, Traykova-Brauch, Hosser, Hähnel, Gröne, Koesters, Kriz: "Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease." in: The American journal of pathology, Vol. 175, Issue 5, pp. 1883-95, (2010) (PubMed).

Choi, Kim, Kwon, Lee, Kang, Kim, Lee, Kwon: "4-1BB functions as a survival factor in dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 7, pp. 4107-15, (2009) (PubMed).

Antigendetails

Target: Macrophages

Hintergrund: Synonyms: Macrophage marker