CD14 Antikörper

Produktnummer ABIN111907

Produktdetails anti-CD14 Antikörper

Target: CD14

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14. BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized.

Aufreinigung: Affinity Chromatography.

Immunogen: Human Monocytes.

Klon: X-8

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Does not react on routinely processed Paraffin Sections. Recommended Positive Control: Human placenta. Has been described to work in FACS (post fixation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL distilled water (= Stock solution).

Konzentration: 0.2 mg/mL

Buffer: PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14 (CD14 Produkte)

Hintergrund: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gen-ID: 929, 9606

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades