Interdigitating Cells (Access. T-Cell Macrophages) Antikörper

Produktnummer ABIN111944

Produktdetails

Target: Interdigitating Cells (Access. T-Cell Macrophages)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Affinity chromatography

Immunogen: Human mononuclear peripheral blood cells.

Klon: X-12

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry on frozen sections (0.25 μg/mL, 1/800) and FACS. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS pH 7.2 containing 0.01 % thiomerosal as preservative and 10 mg/mL BSA as stabilizer.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Antigendetails

Target: Interdigitating Cells (Access. T-Cell Macrophages)