Endothelial Cells Antikörper

Produktnummer ABIN111945

Produktdetails anti-Endothelial Cells Antikörper

Target: Endothelial Cells

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Endothelial Cells Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Affinity A chromatography.

Immunogen: Cultured HUVECs.

Klon: 1F10

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen sections: 2 μg/mL (1: 100),Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigenexpression on cultured HUVEC is dependent on the culture conditions. Best results wereobtained (40-70 % pos. ) by daily renewal of FCS containing medium supplemented withendothelial cell conditioned supernatant whereas commercial available specificendothelial cell media have no influence on the antigen expression (see Table 1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu Endothelial Cells

Target: Endothelial Cells

Abstract: Endothelial Cells Produkte

Hintergrund: Synonyms: Endothelial Cell marker