p53 Antikörper (AA 16-25) (FITC)

Produktnummer ABIN111970

Produktdetails anti-p53 Antikörper (FITC)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: AA 16-25

Reaktivität: Human, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF)

Spezifität: This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.

Immunogen: Bacterially expressed full-length wild-type p53.

Klon: BP53-12

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: This conjugate is routinely tested by Flow Cytometry analysis usingpermeabilized transient wild-type p53-tranfectants.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-p53 Antikörper (FITC) (ABIN111970)

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, (1997) (PubMed).

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, (1991) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53) (TP53 Produkte)

Hintergrund: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gen-ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt