Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.
Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).
Konzentration
0.6 mg/mL
Buffer
PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.
Konservierungsmittel
Kathon CG
Vorsichtsmaßnahmen
This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store (in aliquots) at -20 °C.
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Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule