MHC Class I, H-2 Antikörper

Produktnummer ABIN111986

Produktdetails

Target: MHC Class I, H-2

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Affinity Chromatography.

Immunogen: Mouse macrophage precursor cells Remarks: MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta2-microglobulin (11.5 kDa). The epitope recognized by ER-HR52 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.

Klon: ER-HR52

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 1 μg/mL (1/200). Recommended Positive Control: Mouse Spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!

Antigendetails

Target: MHC Class I, H-2