Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.
Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll
Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 0.5 mL distilled water (=stock solution).