MHC Class II (I-A K,s,r) Antikörper
Kurzübersicht für MHC Class II (I-A K,s,r) Antikörper (ABIN111990)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Aufreinigung
- Affinity Chromatography
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Immunogen
- Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.
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Isotyp
- IgG2b
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Applikationshinweise
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protokoll
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- Restore with 0.5 mL distilled water (=stock solution).
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Konzentration
- 0.2 mg/mL
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Buffer
- Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA
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Konservierungsmittel
- Thimerosal (Merthiolate)
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Vorsichtsmaßnahmen
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2 - 8 °C.
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- MHC Class II (I-A K,s,r)
Target
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