Soluble Terminal Complement Complex (sC5b-9) Antikörper

Produktnummer ABIN112002

Produktdetails

Target: Soluble Terminal Complement Complex (sC5b-9)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Species reactivity (tested):Human: Follicular dendritic (reticulum) cells, C9 neoepitope.No cross reaction observed when tested with polymerized complement C9 of 38 different species, including Mouse, Rat, Rabbit and Guinea Pig.

Aufreinigung: Affinity Chromatography

Immunogen: Enriched Human blood dendritic cells. Remarks: The antigen is a neoepitope of C9 after assembly in the terminal complement complex. Epitope sequence has not been determined.

Klon: X-11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/200). Microwave pretreatment forantigen retrieval is recommended. Has been described ro work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7min. at 700Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 0.1 % Kathon, 5 mg/mL BSA

Konservierungsmittel: Kathon CG

Vorsichtsmaßnahmen: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Antigendetails

Target: Soluble Terminal Complement Complex (sC5b-9)

Andere Bezeichnung: Complement SC5b-9 (TCC / MAC)

Hintergrund: Follicular dendritic cells and sinus lining cells in lymph node and tonsil, and blood dendritic cells express the X-11 antigen. X-11+ blood dendritic cells show typical dendritic veils and are the most potent stimulator cells in an allogenic mixed leukocyte reaction when compared with other leukocytes. X11+ cells strongly react with anti S100 and weakly with anti CD68. The literature suggests that X-11+ cells are dendritic cells of monocytic origin.Synonyms: Marker for Complement C9 Neoepitope On Follicular Dendritic Cells, Membrane attack complex, Terminal complement complex