C3 Antikörper

Produktnummer ABIN112153

Produktdetails anti-C3 Antikörper

Target: C3 (Complement Component 3 (C3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kDa fragment of C3c). Disorders Specifically Detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with Positive Control anti C3b-beta, (H11, Cat. No. BM2132) and Negative Control anti-C3a (H13, Cat. No. BM2131). Activated, deposited C3b bears C3b-alpha and C3b-beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: Human complement component C3

Klon: H206

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunobloting (Western). Immunohistochemistry on Frozen Sections (also after Fixation with Paraformaldehyde): Working Dilution: 1/10 in PBS, pH 7.4. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Details zu C3

Target: C3 (Complement Component 3 (C3))

Andere Bezeichnung: Complement C3 (C3 Produkte)

Synonyme: AHUS5 antikoerper, ARMD9 antikoerper, ASP antikoerper, C3a antikoerper, C3b antikoerper, CPAMD1 antikoerper, AI255234 antikoerper, HSE-MSF antikoerper, Plp antikoerper, C3 antikoerper, C3-1 antikoerper, sb:cb26 antikoerper, si:dkey-76b14.4 antikoerper, wu:fi34b03 antikoerper, wu:fj86e11 antikoerper, C3d antikoerper, asp antikoerper, cpamd1 antikoerper, c3 antikoerper, complement C3 antikoerper, complement component 3 antikoerper, complement component c3a, duplicate 1 antikoerper, complement component 3 L homeolog antikoerper, complement component C3 antikoerper, complement C3-like antikoerper, C3 antikoerper, c3a.1 antikoerper, c3.L antikoerper, LOC443475 antikoerper, c3 antikoerper, c3-1 antikoerper, LOC100060539 antikoerper

Hintergrund: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gen-ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling