C1QA Antikörper

Produktnummer ABIN112267

Produktdetails anti-C1QA Antikörper

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C1QA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Spezifität: Detection of C1q in serum, in serum-C1 and within immune complexes, inhibits hemolytic activity of C1 by binding to C1q in the liquid phase (antibody concentration necessary to inhibit 50 % of hemolytic activity in the fluid phase is 0.85 μg/mL). With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5 μg/mL (Ref.1: Heinz et al, 1984). Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders. Polypetide Reacting: C-chain of complement C1q (25 kDa in SDS-PAGE).

Aufreinigung: Affinity Chromatography on Protein A .

Immunogen: Purified human complement C1q.

Klon: 242G3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Inhibition experiments (e. g. hemolytic activity). Immunoblotting (Western): 1/100, 1h at RT (ECL method).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water.

Buffer: Final Solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Details zu C1QA

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Andere Bezeichnung: C1q A Subunit (C1QA Produkte)

Hintergrund: C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with the tetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and is composed of three basic subunits termed A, B and C (27,550, 25,200, 23,800 Da respectively) which asociate in the proportion 6 A-B subunits (52, 750 Da) and 3 C-C subunits (47,600 Da), to form whole C1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation.Synonyms: Complement 1q, Complement C1q

Gen-ID: 712

NCBI Accession: NP_057075

UniProt: P02745

Pathways: Komplementsystem