KRT8 Antikörper

Produktnummer ABIN112350

Produktdetails anti-KRT8 Antikörper

Target: KRT8 (Keratin 8 (KRT8))

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This Monoclonal antibody H1 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Zebrafish.

Aufreinigung: Purified

Immunogen: Cytoskeleton preparation containing Cytokeratin 8.

Klon: H1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting, Immunocytochemistry, immunohistochemistry on frozen andparaffin-embedded tissues and flow cytometry. Recommended dilutions: 1/25-1/200 for flow cytometry, and for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.

Details zu KRT8

Target: KRT8 (Keratin 8 (KRT8))

Andere Bezeichnung: Cytokeratin 8 (KRT8 Produkte)

Hintergrund: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gen-ID: 3856

NCBI Accession: NP_002264

UniProt: P05787