OB Cadherin Antikörper (Extracellular Domain)

Produktnummer ABIN112366

Produktdetails anti-OB Cadherin Antikörper

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OB Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Spezifität: This antibody recognizes the extracellular domain of Cadherin-11.

Aufreinigung: Affinity Chromatography

Immunogen: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with affinity purified extracellular domain of Human Cadherin-11-GST fusion protein.

Klon: 16A

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting, Immunocytochemistry and Immunohistochemistry on Frozensections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended Dilutions: 1/25-1/50 for Immunohistochemistry with Avidin-biotinylatedHorseradish Peroxidase Complex (ABC) as detection reagent.1/25-1/250 for Immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the original vial at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Andere Bezeichnung: Cadherin-11 (CDH11 Produkte)

Hintergrund: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.Synonyms: CDH11, OB Cadherin, OSF-4, Osteoblast cadherin

Gen-ID: 1009

NCBI Accession: NP_001788

UniProt: P55287