The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
Flow CytometryDilution: 1 mg/mLPositive control: PBL cell line,Negative control: DAUDI human lymphoma cell line, erythrocytes. ImmunoprecipitationB2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from thelysates of human lymphoid cells. RIAThe dissociation constant of the antibody soluble beta2-Microglobulin is 1.5 x 10e-8 Mol/las determined by competitive RIA. ELISA. Immunohistochemistry (Paraffin)Dilution: 10 μg/mL. Positive tissue: kidney glomeruli. Western blotting (Reducing and Non-Reducing Conditions). Dilution: 2-4 μg/mL. Positive control: U937 human histiocytic ymphoma cell line. Negative control: EL4 mouse lymphoblastic lymphoma cell line. Sample Preparation (See Protocols).
Protokoll
Sample Preparation (Western Blotting): Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mMTris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Protease inhibitor Cocktail). Incubate60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducingLaemmli SDS-PAGE sample buffer.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin