MAP2 Antikörper

Produktnummer ABIN112902

Produktdetails anti-MAP2 Antikörper

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Prepared by Ammonium Sulphate fractionation

Immunogen: Native, from brain

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections (1: 5 - 1: 30). Western blot (Recognises mainly the MAP2 protein (280 kD) and to a lesser extent the Tauprotein (60 kD) in blotting using bovine brain extract. When testing against rat brainextract this antibody shows strong bands at 60 kD and fainter bands at the highermolecular weights. This could be due to differences in the sample preparation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 25.5 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Abstract: MAP2 Produkte

Synonyme: MAP2A antikoerper, MAP2B antikoerper, MAP2C antikoerper, MAP2R antikoerper, Mtap2 antikoerper, MAP-2 antikoerper, Mtap-2 antikoerper, xmap2 antikoerper, MGC53131 antikoerper, a730034c02 antikoerper, zgc:103474 antikoerper, zgc:113974 antikoerper, microtubule associated protein 2 antikoerper, microtubule-associated protein 2 antikoerper, microtubule associated protein 2 S homeolog antikoerper, MAP2 antikoerper, Map2 antikoerper, map2.S antikoerper, map2 antikoerper

Hintergrund: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.