AMY1
Reaktivität: Human
WB, IHC, ICC, IF
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Immunohistochemistry (frozen): 1/50 - 1/200. ELISA (In a simple indirect ELISA with 400 ng of human salivary amylase bound to theplate, the 50 % OD was observed at an antibody dilution of 1/5K. Under the sameconditions, the end point was observed at 1/500K). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
5.0 mg/mL
Buffer
PBS pH 7.2 containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary starch and glycogen. Alternative splicing results in multiple transcript variants encoding the same protein. Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56 kDa (family B) or 62 kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50 % is salivary amylase. Although salivary amylase and pancreatic amylase share 95 % amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.Synonyms: 1, 4-alpha-D-glucan glucanohydrolase 1, AMY1, AMY1A, Salivary alpha-amylase