This anti-mouse CD8 b-Chain monoclonal antibody is specific for most thymocytes, cytotoxic/suppressor T-cells and their precursors. The CD8 b-Chain is also named Ly-3.
Flow Cytometry Analysis (see Protocols). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll
FLOW CYTOMETRY ANALYSIS:
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.2 mg/mL
Buffer
PBS and 0.1 % NaN3
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain