CD72 Antikörper (PE)

Produktnummer ABIN114241

Produktdetails anti-CD72 Antikörper (PE)

Target: CD72 (CD72 Molecule (CD72))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD72 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: This monoclonal antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells1. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J.

Klon: CT-72-1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 25 µg/10e6 cellsIsotypic Control: PE Mouse IgG2aStrain Tested: DBA mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu CD72

Target: CD72 (CD72 Molecule (CD72))

Andere Bezeichnung: CD72 (CD72 Produkte)

Hintergrund: CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32

Gen-ID: 100504743

NCBI Accession: XP_003086403

UniProt: P21855

Pathways: BCR Signaling