MHC Class II I-Abd Antikörper (Biotin)

Produktnummer ABIN114307

Produktdetails

Target: MHC Class II I-Abd

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS)

Aufreinigung: Affinity chromatography

Klon: 28-16-8S

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2µg - 0. 5mg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS and contains 0.02 % sodium azide (NaN3) as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class II I-Abd