MHC Class II I-Abd Antikörper

Produktnummer ABIN114309

Produktdetails

Target: MHC Class II I-Abd

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Cytotoxicity Test (CyTox)

Aufreinigung: Affinity chromatography

Immunogen: C3H.SW spleen Donor: C3H Fusion Partner: SP2/0Ag.14

Klon: 28-16-8S

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Can be used to quantitate or eliminate cells bearing the I-Ab antigen from the appropriatestrains of mice.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1. 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 640 and mix.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS and contains 0.02 % sodium azide (NaN3) as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: MHC Class II I-Abd