MHC Class II I-Ek Antikörper

Produktnummer ABIN114319

Produktdetails anti-MHC Class II I-Ek Antikörper

Target: MHC Class II I-Ek

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II I-Ek Antikörper ist unkonjugiert

Applikation: Cytotoxicity Test (CyTox)

Aufreinigung: Protein G chromatography

Immunogen: C3H Donor: C3H.SW Fusion Partner: SP2/0-Ag 14

Klon: 14-4-4S

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS and 0.02 % NaN3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MHC Class II I-Ek

Target: MHC Class II I-Ek

Abstract: MHC Class II I-Ek Produkte