Endothelium Antikörper

Produktnummer ABIN114384

Produktdetails

Target: Endothelium

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Rat peritoneal macrophages

Klon: OX-43

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (10 l of 1: 50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. ). Immunohistochemistry on frozen sections. Immunprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Endothelium

Hintergrund: The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.Synonyms: endothelial cells, endothelial marker