MHC Class I (RT1Ac) Antikörper (FITC)

Produktnummer ABIN114394

Produktdetails anti-MHC Class I (RT1Ac) Antikörper (FITC)

Target: MHC Class I (RT1Ac)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class I (RT1Ac) Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Aufreinigung: Protein G Chromatography.

Immunogen: Phytohaemagglutinin Blasts.

Klon: OX-27

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use 0.5 g of neat antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Recognizes a polymorphic determinant of the MHC class I antigen in the rat. This Antibody can be used for labelling cells of donor or host origin in bone marrowchimeras. (1,2)

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS buffer with 0.02 % sodium azide as preservative and 0.5 % EIA grade BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu MHC Class I (RT1Ac)

Target: MHC Class I (RT1Ac)

Abstract: MHC Class I (RT1Ac) Produkte

Hintergrund: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3

Gen-ID: 10116

UniProt: Q31255