Clone RCK108 specifically reacts with Cytokeratin 19, found in most glandular simple type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma.
Aufreinigung
Purified
Immunogen
Cytoskeletal preparation of Human bladder carcinoma cell line T24
Immunohistochemistry- on frozen sections. - on paraffin embedded tissues (pre-treatment with pepsin or trypsin). Immunocytochemistry. Immunoblotting. Flow cytometry: 1: 100 - 1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Individual human cytokeratins are numbered 1 to 20.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19