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His Tag Antikörper

WB Wirt: Kaninchen Polyclonal unconjugated

Produktnummer ABIN115691

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag

Reaktivität

Bitte anfragen
Wirt
94

60

11

9

5

4

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
96

76

10
Polyklonal
Konjugat
79

22

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
143

100

39

27

26

19

15

14

14

13

13

11

7

5

5

4

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB)

Sequenz

HHHHHH

Aufreinigung

Antigen Affinity Chromatography using a His-peptide as matrix.

Immunogen

Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.

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anti-His Tag antibody
Reaktivität: Human WB Wirt: Maus Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Wirt: Maus Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Wirt: Maus Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Wirt: Maus Monoclonal 33D10-D2 HRP

anti-His Tag antibody
Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB19960 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Wirt: Maus Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt Wirt: Maus Monoclonal HIS-H8 unconjugated

Applikationshinweise

Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Rekonstitution

Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer

PBS, pH 7.4, without preservatives.

Konservierungsmittel

Without preservative

Handhabung

Avoid repeated freezing and thawing.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.

Haltbarkeit

12 months
Target

His Tag

Andere Bezeichnung

6xHistidine Epitope Tag (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called