XCL1
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhLymphotactin (60 ng/mL), a concentration of 1.7 - 3.0 μg/mL of this antibody is required. ELISA: To detect hLymphotactin by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hLymphotactin. Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLymphotactin is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.