CCL23
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: To detect hMIP-3 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.25 - 1.0 μg/mL. Used in conjunction with compatible secondaryreagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3. To detect hMIP-3 by sandwich ELISA (using 100 l/well antibody solution) a concentrationof 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, inconjuction with anti-human MIP-3 (cat-no. PP1089P1/2) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant human MIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Centrifuge the vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antibody is stable at -20 °C for one year from date of despatch. Reconstituted antibody is stable for 2 weeks at 2-8 °C and for six months when stored in working aliquots at -20 °C.
Macrophage Inflammatory Protein 3 (MIP3) is chemotactic for monocytes, resting T-lymphocytes, and neutrophils. It inhibits proliferation of myeloid progenitor cells and is highly expressed in adult lung, liver, skeletal muscle and pancreas. There are moderate levels of MIP3 in fetal liver, adult bone marrow and placenta. MIP3 belongs to the intercrine beta family (small cytokine c-c) (chemokine c-c).Synonyms: C-C motif chemokine 23, CCL-23, CK-beta-8, CKB-8, MIP-3, MPIF1, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, SCYA23, Small-inducible cytokine A23