Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.
Aufreinigung
Prepared from monospecific antiserum by a delipidation and defibrination.
CPA1
Reaktivität: Human
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Suitable for Immunoblotting (Western blot: 1/500-1/3,000or Dot blot), ELISA (1/5,000-1/25,000), Immunoprecipitation and most immunologicalmethods requiring high titer and specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6, without preservative.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
Carboxypeptidase A is a pancreatic exopeptidase. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties.Synonyms: CPA, CPA1