HRP Antikörper

Produktnummer ABIN116806

Produktdetails anti-HRP Antikörper

Target: HRP (Horseradish Peroxidase (HRP))

Reaktivität: Meerrettich

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Prepared from monospecific antiserum by a Delipidation and Defibrination

Immunogen: Peroxidase from Horseradish.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot, 1/2,000-1/10,000), ELISA(1/20,000-1/100,000), Immunoprecipitation, Immunohistochemistry (1/1,000-1/5,000)and most Immunological methods requiring high titer and specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.2, None, None

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use. . Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Details zu HRP

Target: HRP (Horseradish Peroxidase (HRP))

Andere Bezeichnung: Horseradish Peroxidase / HRP (HRP Produkte)

Hintergrund: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.