Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Spezifität
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
Aufreinigung
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended dilutions: ELISA: 1/30,000-1/150,000. Western blot: 1/3,000-1/15,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with no preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target
RNASE1
(Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))