Suitable for immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 μg of XanthineOxidase [Bovine Buttermilk] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 100,000 to 1: 500,000 of the reconstitutionconcentration is suggested for this product. This product has been assayed by Immunhistochemical staining and was found to bereactive against bovine, human and mouse tissue at a dilution of 1: 5,000 to 1: 25,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
85.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01 % sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
Robenek, Hofnagel, Buers, Lorkowski, Schnoor, Robenek, Heid, Troyer, Severs: "Butyrophilin controls milk fat globule secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10385-10390, (2006) (PubMed).