Laminin Antikörper (Biotin)

Produktnummer ABIN116904

Dieses Anti-Laminin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Laminin in WB, IP, IHC (p), IHC (fro) und EIA. Geeignet für Human.

Kurzübersicht für Laminin Antikörper (Biotin) (ABIN116904)

Target: Laminin (LN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Laminin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Laminin Antikörper (Biotin)

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Anwendungsinformationen

Applikationshinweise: Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Laminin

Target: Laminin (LN)

Andere Bezeichnung: Laminin