This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
Aufreinigung
Protein A chromatography.
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
LEFTY2
Reaktivität: Human
WB, ELISA
Wirt: Maus
Monoclonal
1H6
unconjugated
Applikationshinweise
This antibody is suitable for ELISA (1/5,000) and Western blotting (1/2,000). The antibodymay be used for other applications, such as RIA, Immunohistochemistry orImmunoprecipitation, but specific reaction conditions have not been developed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: EBAF, Endometrial bleeding-associated factor, LEFTA, LEFTY2, LEFTYA, Left-right determination factor 2, Left-right determination factor A, Protein lefty-2, Protein lefty-A, TGF-beta-4, TGFB4, Transforming growth factor beta-4