Rekombinanter Tubulin-GTP Antikörper

Produktnummer ABIN1169388

Produktdetails

Target: Tubulin-GTP

Antikörpertyp: Recombinant Antibody

Reaktivität: Drosophila melanogaster, Human, Maus, Ratte

Wirt: Human

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC)

Spezifität: Recognizes human, mouse, rat and drosophila tubulin-GTP.

Kreuzreaktivität: Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus)

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Full length GTP-lambda-S loaded tubulin from pig brain.

Klon: MB11

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen

Van Steenbergen, Boesmans, Li, de Coene, Vints, Baatsen, Dewachter, Ameloot, Clays, Vanden Berghe: "Molecular understanding of label-free second harmonic imaging of microtubules." in: Nature communications, Vol. 10, Issue 1, pp. 3530, (2019) (PubMed).

Nakata, Niwa, Okada, Perez, Hirokawa: "Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 245-55, (2011) (PubMed).

Dimitrov, Quesnoit, Moutel, Cantaloube, Poüs, Perez: "Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues." in: Science (New York, N.Y.), Vol. 322, Issue 5906, pp. 1353-6, (2008) (PubMed).

Antigendetails

Target: Tubulin-GTP

Hintergrund: Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.