This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of Mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions.
Aufreinigung
Protein A chromatography
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
This antibody is suitable for use in Neutralizations, ELISA, Immunohistochemistry andImmunoblotting. Although untested, this reagent may be useful for Radioimmunoassays,Flow cytometry and Immunoprecipitation. It recognizes the 18,000 MW mature (active)IL-18. Reactivity in other Immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRP
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing no stabilizers or preservatives
Konservierungsmittel
Azide free
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Sakai, Sugawara, Kuroishi, Sasano, Sugawara: "Identification of IL-18 and Th17 cells in salivary glands of patients with Sjögren's syndrome, and amplification of IL-17-mediated secretion of inflammatory cytokines from salivary gland cells by IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 4, pp. 2898-906, (2008) (PubMed).
Lauritsen, Madsen: "Synthesis of naturally occurring iminosugars from D-fructose by the use of a zinc-mediated fragmentation reaction." in: Organic & biomolecular chemistry, Vol. 4, Issue 15, pp. 2898-905, (2006) (PubMed).
Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64 % aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91 % aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma