ATG12 Antikörper

Produktnummer ABIN116953

Produktdetails anti-ATG12 Antikörper

Target: ATG12 (Autophagy Related 12 (ATG12))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Kreuzreaktivität (Details): Species reactivity (tested):S. cerevisiae, Yeast

Aufreinigung: A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Anwendungsinformationen

Applikationshinweise: This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water or equivalent.

Konzentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Andere Bezeichnung: ATG12 / APG12 (ATG12 Produkte)

Synonyme: APG12 antikoerper, APG12L antikoerper, FBR93 antikoerper, HAPG12 antikoerper, Apg12l antikoerper, AUTOPHAGY 12 antikoerper, AUTOPHAGY 12 B antikoerper, CG10861 antikoerper, Dmel\\CG10861 antikoerper, atg12 antikoerper, ATG12 antikoerper, apg12 antikoerper, DDBDRAFT_0185323 antikoerper, DDBDRAFT_0191412 antikoerper, DDB_0185323 antikoerper, DDB_0191412 antikoerper, 4931423H11Rik antikoerper, A330058M13Rik antikoerper, Atg12l antikoerper, AO090001000593 antikoerper, autophagy related 12 antikoerper, Ubiquitin-like superfamily protein antikoerper, Atg12p antikoerper, autophagy protein antikoerper, ubiquitin-like protein ATG12B antikoerper, autophagy associated protein Atg12 antikoerper, Autophagy-related 12 antikoerper, ATG12 autophagy related 12 homolog (S. cerevisiae) antikoerper, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene antikoerper, autophagy associated ubiquitin-like modifier Atg12 antikoerper, hypothetical protein antikoerper, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) antikoerper, autophagy protein 12 antikoerper, autophagy related 12 S homeolog antikoerper, autophagy-related protein 12 antikoerper, ATG12 antikoerper, Atg12 antikoerper, APG12B antikoerper, APG12 antikoerper, LOC9321066 antikoerper, apg12 antikoerper, atg12 antikoerper, LOC743425 antikoerper, CAALFM_C113950CA antikoerper, atg12.S antikoerper, ANI_1_922094 antikoerper, AOR_1_1064164 antikoerper

Hintergrund: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

Gen-ID: 852518

NCBI Accession: NP_009776

UniProt: P38316

Pathways: Autophagie