CD44 Antikörper

Produktnummer ABIN1176842

Produktdetails anti-CD44 Antikörper

Target: CD44

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2

Klon: KM114

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD44 Antikörper (ABIN1176842)

Castro, Bono, Simon, Vargas, Rosemblatt: "Spleen-derived stromal cells. Adhesion molecules expression and lymphocyte adhesion to reticular cells." in: European journal of cell biology, Vol. 74, Issue 4, pp. 321-8, (1998) (PubMed).

Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." in: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, (1997) (PubMed).

Kanamori, Ishimaru, Nanno, Maki, Ikuta, Nariuchi, Ishikawa: "Identification of novel lymphoid tissues in murine intestinal mucosa where clusters of c-kit+ IL-7R+ Thy1+ lympho-hemopoietic progenitors develop." in: The Journal of experimental medicine, Vol. 184, Issue 4, pp. 1449-59, (1996) (PubMed).

Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." in: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, (1996) (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, (1995) (PubMed).

Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, (1995) (PubMed).

Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, (1994) (PubMed).

Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, (1994) (PubMed).

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

Murakami, Miyake, June, Kincade, Hodes: "IL-5 induces a Pgp-1 (CD44) bright B cell subpopulation that is highly enriched in proliferative and Ig secretory activity and binds to hyaluronate." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3618-27, (1991) (PubMed).

MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." in: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, (1990) (PubMed).

Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, (1990) (PubMed).

Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, (1989) (PubMed).

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44 (Pgp-1, H-CAM, Ly24) (CD44 Produkte)

Synonyme: CDW44 antikoerper, CSPG8 antikoerper, ECMR-III antikoerper, HCELL antikoerper, HUTCH-I antikoerper, IN antikoerper, LHR antikoerper, MC56 antikoerper, MDU2 antikoerper, MDU3 antikoerper, MIC4 antikoerper, Pgp1 antikoerper, AU023126 antikoerper, AW121933 antikoerper, AW146109 antikoerper, HERMES antikoerper, Ly-24 antikoerper, Pgp-1 antikoerper, CD44A antikoerper, METAA antikoerper, RHAMM antikoerper, XCD44 antikoerper, cd44 antikoerper, cdw44 antikoerper, cspg8 antikoerper, ecmr-iii antikoerper, hcell antikoerper, lhr antikoerper, mc56 antikoerper, mdu2 antikoerper, mdu3 antikoerper, mic4 antikoerper, mutch-i antikoerper, pgp1 antikoerper, CD44 molecule (Indian blood group) antikoerper, syndecan 4 antikoerper, CD44 antigen antikoerper, CD44 molecule (Indian blood group) S homeolog antikoerper, CD44 antikoerper, SDC4 antikoerper, Cd44 antikoerper, cd44.S antikoerper

Hintergrund: The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7. The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, and memory cells can be recognized by their CD44[hi] phenotype. KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44. It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus, but not human CD44.
Synonyms: Pgp-1, H-CAM, Ly-24

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling