BTK Antikörper (N-Term) (PE)

Produktnummer ABIN1176914

Der Maus Monoklonal Anti-BTK-Antikörper wurde für ICS validiert. Er ist geeignet, BTK in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für BTK Antikörper (N-Term) (PE) (ABIN1176914)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BTK Antikörper ist konjugiert mit PE

Applikation: Intracellular Staining (ICS)

Klon: 53-BTK

Produktdetails anti-BTK Antikörper (PE)

Bindungsspezifität: AA 2-172, N-Term

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-Terminal Btk aa. 2-172 Recombinant Protein

Isotyp: IgG2a

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-BTK Antikörper (PE) (ABIN1176914)

Marshall, Niiro, Yun, Clark: "Regulation of B-cell activation and differentiation by the phosphatidylinositol 3-kinase and phospholipase Cgamma pathway." in: Immunological reviews, Vol. 176, pp. 30-46, (2001) (PubMed).

Rawlings, Scharenberg, Park, Wahl, Lin, Kato, Fluckiger, Witte, Kinet: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, (1996) (PubMed).

Mahajan, Fargnoli, Burkhardt, Kut, Saouaf, Bolen: "Src family protein tyrosine kinases induce autoactivation of Bruton's tyrosine kinase." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5304-11, (1995) (PubMed).

Details zu BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Andere Bezeichnung: Btk

Hintergrund: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. It is a member of the Tec family of kinases which includes Tec and Itk. This family is similar to the src family of tyrosine kinases. However, Tec family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain. The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551. This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The PH domain is essential for proper activation and function of Btk. A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

Pathways: Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling