ZAP70 Antikörper (pTyr319) (PerCP-Cy5.5)

Produktnummer ABIN1176924

Dieses Anti-ZAP70-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ZAP70 in ICS. Geeignet für Human. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für ZAP70 Antikörper (pTyr319) (PerCP-Cy5.5) (ABIN1176924)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist konjugiert mit PerCP-Cy5.5

Applikation: Intracellular Staining (ICS)

Klon: 17A-P

Produktdetails anti-ZAP70 Antikörper (PerCP-Cy5.5)

Bindungsspezifität: pTyr319

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human phosphorylated ZAP70 Peptide

Isotyp: IgG1

Anwendungsinformationen

Probenmenge: 5 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

Referenzen für anti-ZAP70 Antikörper (PerCP-Cy5.5) (ABIN1176924)

Di Bartolo, Mège, Germain, Pelosi, Dufour, Michel, Magistrelli, Isacchi, Acuto: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, (1999) (PubMed).

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, (1994) (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, (1994) (PubMed).

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund: ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling. In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319). It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319). The PE-conjugated format has been evaluated using human and mouse model systems. The unconjugated form of the antibody (Cat. No. 612574) has also been shown to work in western blot analysis on human, mouse, and rat cells.
Synonyms: ZAP-70, SRK, STD, TZK, Zeta-chain associated protein kinase, 70kD

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway