SELPLG Antikörper

Produktnummer ABIN1177261

Produktdetails anti-SELPLG Antikörper

Target: SELPLG (Selectin P Ligand (SELPLG))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser SELPLG Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Reagent (BR)

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: PSGL-1 human IgG1 fusion protein

Klon: 4RA10

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-SELPLG Antikörper (ABIN1177261)

Phillips, Barringhaus, Sanders, Hesselbacher, Czarnik, Manka, Vestweber, Ley, Sarembock: "Single injection of P-selectin or P-selectin glycoprotein ligand-1 monoclonal antibody blocks neointima formation after arterial injury in apolipoprotein E-deficient mice." in: Circulation, Vol. 107, Issue 17, pp. 2244-9, (2003) (PubMed).

Sperandio, Smith, Forlow, Olson, Xia, McEver, Ley: "P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules." in: The Journal of experimental medicine, Vol. 197, Issue 10, pp. 1355-63, (2003) (PubMed).

Xia, Sperandio, Yago, McDaniel, Cummings, Pearson-White, Ley, McEver: "P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow." in: The Journal of clinical investigation, Vol. 109, Issue 7, pp. 939-50, (2002) (PubMed).

Hirata, Furie, Furie: "P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 8, pp. 4307-13, (2002) (PubMed).

Pendl, Robert, Steinert, Thanos, Eytner, Borges, Wild, Lowe, Fuhlbrigge, Kupper, Vestweber, Grabbe: "Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1." in: Blood, Vol. 99, Issue 3, pp. 946-56, (2002) (PubMed).

Hirata, Merrill-Skoloff, Aab, Yang, Furie, Furie: "P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1669-76, (2000) (PubMed).

Frenette, Denis, Weiss, Jurk, Subbarao, Kehrel, Hartwig, Vestweber, Wagner: "P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo." in: The Journal of experimental medicine, Vol. 191, Issue 8, pp. 1413-22, (2000) (PubMed).

Borges, Eytner, Moll, Steegmaier, Campbell, Ley, Mossmann, Vestweber: "The P-selectin glycoprotein ligand-1 is important for recruitment of neutrophils into inflamed mouse peritoneum." in: Blood, Vol. 90, Issue 5, pp. 1934-42, (1997) (PubMed).

Borges, Tietz, Steegmaier, Moll, Hallmann, Hamann, Vestweber: "P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 573-8, (1997) (PubMed).

Steegmaier, Blanks, Borges, Vestweber: "P-selectin glycoprotein ligand-1 mediates rolling of mouse bone marrow-derived mast cells on P-selectin but not efficiently on E-selectin." in: European journal of immunology, Vol. 27, Issue 6, pp. 1339-45, (1997) (PubMed).

Li, Wilkins, Crawley, Weinstein, Cummings, McEver: "Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin." in: The Journal of biological chemistry, Vol. 271, Issue 6, pp. 3255-64, (1996) (PubMed).

Yang, Galipeau, Kozak, Furie, Furie: "Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor." in: Blood, Vol. 87, Issue 10, pp. 4176-86, (1996) (PubMed).

Details zu SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Andere Bezeichnung: CD162 (SELPLG Produkte)

Synonyme: CD162 antikoerper, CLA antikoerper, PSGL-1 antikoerper, PSGL1 antikoerper, SELPLG antikoerper, SELPG antikoerper, Psgl-1 antikoerper, Psgl1 antikoerper, Selp1 antikoerper, Selpl antikoerper, selectin P ligand antikoerper, selectin, platelet (p-selectin) ligand antikoerper, clasper antikoerper, SELPLG antikoerper, Selplg antikoerper, cla antikoerper

Hintergrund: The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
Synonyms: PSGL-1