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CD28 Antikörper

Der Maus Monoklonal Anti-CD28-Antikörper wurde für FACS und BCA validiert. Er ist geeignet, CD28 in Proben von Human zu detektieren.
Produktnummer ABIN1177331

Kurzübersicht für CD28 Antikörper (ABIN1177331)

Target

Alle CD28 Antikörper anzeigen
CD28

Reaktivität

  • 215
  • 120
  • 55
  • 38
  • 22
  • 17
  • 13
  • 12
  • 7
  • 7
  • 7
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 182
  • 83
  • 33
  • 12
  • 10
  • 3
  • 2
  • 1
  • 1
Maus

Klonalität

  • 246
  • 74
  • 4
Monoklonal

Konjugat

  • 128
  • 31
  • 28
  • 22
  • 15
  • 7
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD28 Antikörper ist unkonjugiert

Applikation

  • 233
  • 101
  • 72
  • 52
  • 46
  • 43
  • 37
  • 28
  • 26
  • 23
  • 13
  • 7
  • 7
  • 6
  • 6
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Biochemical Assay (BCA)

Klon

CD28-2
  • Marke

    BD Pharmingen™

    Aufreinigung

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Sterilität

    0.2 μm filtered

    Endotoxin-Niveau

    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

    Immunogen

    Human CD28 Transfected Cell Line

    Isotyp

    IgG1 kappa
  • Protokoll

    1. Isolate human PBMC with Ficoll-Paque™ Plus
    2. Suspend PBMC in medium supplemented with 1% glutamine, 1% penicillin/streptomycin and 10% FBS at 10^6 cells/ml
    3. Incubate 1 x 10^6 PBMC with soluble NA/LE™ format of CD3 mAb (Cat. No. 555336) at 1µg/ml final concentration in 7% CO2, 37°C for 3 days
    4. Wash cells twice and resuspend in the above medium at 10^6 cells/ml
    5. Distribute cells in 96-well round bottom plate at 10^5 cells/well
    6. Add soluble NA/LE™ format of CD28.2 mAb at 5 µg/ml final concentration
    7. Incubate the plate for an additional three days 8. Pulse the plate with [3H]TdR (1 µCi/well). Harvest the plate 20 hours after pulse and count
    Comment: Addition of Protein G (Sigma) 5 µg/ml together with CD28.2 will significantly enhance the costimulation, presumably through cross-linking.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

    Konservierungsmittel

    Azide free

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Target

    CD28

    Andere Bezeichnung

    CD28

    Hintergrund

    The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.
    Synonyms: CD28 antigen, T44, Tp44, TP44

    Pathways

    T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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